Banca de DEFESA: ANDERSON PAULO RODRIGUES LOPES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANDERSON PAULO RODRIGUES LOPES
DATA : 29/04/2022
HORA: 10:00
LOCAL: https://meet.google.com/aej-xqxq-jcy
TÍTULO:

ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY OF ESSENTIAL OIL FROM Lippia gracilis (Verbenaceae) AND OF A NATURAL OINTMENT ON Staphylococcus aureus STRAINS

PALAVRAS-CHAVES:

Biofilm; essential oil; Lippia gracilis; natural ointment.

PÁGINAS: 60
GRANDE ÁREA: Outra
ÁREA: Ciências Ambientais
RESUMO:

Antimicrobial resistance (AMR) has emerged as one of the main health problems due to the high rates of infections caused by bacteria, parasites, viruses or fungi. In addition, microorganisms have a very efficient defense mechanism, the formation of biofilms. In this sense, the discovery of new bioactive products that have antimicrobial activity is extremely relevant to overcome the current problems of antibiotic resistance. Thus, the aim of this study was to evaluate the antimicrobial and antibiofilm activity of essential oil extracted from Lippia gracilis, associated with a natural ointment and also with the antibiotic ampicillin, against different strains of Staphylococcus aureus (ATCC 25923, ATCC 700698 and JKD6008). The antimicrobial activity of the L. gracilis oil, the ointment and the association between them was evaluated in microdilution assays in plates through the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The MIC values of the essential oil were used in combination with the antibiotic ampicillin and its effects were evaluated using the checkerboard technique. Antibiofilm activity was evaluated by quantification of biofilm biomass by crystal violet (CV) staining and by the number of viable biofilm cells by colony forming units (CFU) count. The essential oil presented MIC at 2.5 mg/mL for the three strains of S. aureus and CBM only for S. aureus JKD6008 at the same concentration. The ointment, on the other hand, showed no antimicrobial activity on the three strains tested. The association of the essential oil with the ointment resulted in MIC for S. aureus ATCC 25923 at 2.5 mg/mL and 1.25 mg/mL for S. aureus ATCC 700698 and S. aureus JKD6008. Furthermore, they showed CBM at 5 mg/mL in S. aureus ATCC 25923 and ATCC 700698. The synergistic effect against S. aureus ATCC 25923 (ICIF=0.375), S. aureus ATCC 700698 (ICIF=0.375) strains. Regarding antibiofilm activity, the essential oil of L. gracilis, the ointment and the association between oil and ointment were able to significantly reduce the formation of biofilms and preformed biofilms. In conclusion, the essential oil of L. gracilis showed antimicrobial activity against different strains of S. aureus and their biofilms, either alone or associated with a natural ointment and also with ampicillin.

MEMBROS DA BANCA:
Externo à Instituição - EDSON HOLANDA TEIXEIRA - UFC
Externo ao Programa - 12220 - DIEGO NATHAN DO NASCIMENTO SOUZA
Presidente - 023.053.853-31 - MAYRON ALVES DE VASCONCELOS - UFC