ANTIMICROBIAL ACTIVITY OF PROTEINS EXTRACTED FROM SEEDS OF Luffa operculata
Vegetable proteins; ROS; Biotechnological potential; Vegetable extracts.
Luffa operculata is a vine species native to Brazil widely used for the treatment of respiratory diseases, howerver, studies indicate its effectiveness in the treatment of other diseases. The interest in plant species that have biotechnological applications is of great interest to society, especially when it comes to regional species. Thus, this research aimed to evaluate the antimicrobial potential of protein extracts from Luffa operculata seeds against fungi of the genus Candida. In this perspective, seeds of the species were crushed to form the protein meal that originated the extracts. From these extracts, the parameters were evaluated: protein protein profile; amount of soluble proteins; rate of growth inhibition of microorganisms; aspects that induced the death of fungal cells. Test samples were used in triplicate. The data obtained were submitted to analysis of variance, followed by Tukey's test, using the GraphPad Prism, with a significance of p < 0.05. The results proved that L. operculata proteins have a high antifungal activity against C. albicans and C. krusei fungi. This information can be confirmed when analyzing the rate of inhibition of fungal growth for both species analyzed, as well as through SEM images that demonstrate the effects at the cellular level of the antifungal action, indicating this plant species as a very viable environmental resource and with great potential for the treatment of diseases caused by C. albicans and C. krusei.